- Operates in both directions
- Lido*
- Giardini*
- San Zaccaria*
- Vallaresso (for Piazza San Marco)
- Accademia
- San Samuele
- San Toma'
- Rialto
- San Marcuola
- Ferrovia (railway station)
- Piazzale Roma
- Tronchetto
- Sacca Fisola
- San Basilio
- Zattere
- Palanca
- Redentore
- Zitelle
- San Giorgio
- San Zaccaria
* Summer only. At certain times the line only runs from S Zaccaria to Rialto.